Product Highlight

• Configuration-specific monoclonal antibody that recognizes Arl3-GTP, but not Arl3-GDP
• The only available Arl3 activation assay kit directly measures the level of Arl3-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Arl3 in cells and in tissues by immunohistochemistry

Product Description

Arl3 (Arf-like 3) is an ADP-ribosylation factors (Arf) family protein. Arl3 interacts with and regulates activities of several effector proteins, such as human retinal gene 4 (HRG4), δ-subunit of the cGMP phosphodiesterase (PDEδ), and binder of Arl2 (BART). Arl3 also binds microtubules to alter specific aspects of cytokinesis via interactions with retinitis pigmentosa 2 (RP2).

Currently there is no direct assay to measure the activation of Arl3 GTPases.

NewEast Biosciences Arl3 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arl3-GTP, but not Arl3-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti-Arl3-GTP monoclonal antibodies can also be used to monitor the activation of Arl3 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Arl3 Activation Assay Kit provides a simple and fast method to monitor the activation of Arl3. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Arl3 Activation Assay Kit bases on the configuration-specific anti-Arl3-GTP monoclonal antibody to measure the active Arl3-GTP levels, either from cell extracts or from in vitro GTPγS loading Arl3 activation assays. Briefly, anti-active Arl3 mouse monoclonal antibody will be incubated with cell lysates containing Arl3-GTP. The bound active Arl3 will then be pulled down by protein A/G agarose. The precipitated active Arl3 will be detected by immunoblot analysis using anti- Arl3 rabbit polyclonal antibody.