Anti-BRAF (V600A) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-BRAF (V600A) Mouse Monoclonal Antibody

Anti-BRAF (V600A) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-BRAF (V600A) Mouse Monoclonal Antibody

英文名称： Anti-BRAF (V600A) Mouse Monoclonal Antibody

产品编号： 26199

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司
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BRAF (V600A) [26199]
[100UL]		  

Catalog Number:  26199
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRAF (V600A) Mouse Monoclonal Antibody
Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of V600A, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes V600A mutant, but not wild-type BRAF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF (V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with anti-BRAF (V600A) monoclonal antibody (Cat. #26199).
Immunofluorescence:  
Immunofluorescence of cells expressing BRAF proteins with anti-BRAF (V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF (V600A) plasmid, pCDNA3-GFP-BRAF (V600D) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid pCDNA3-GFP-BRAF (V600M) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid, then fixed and stained with anti-BRAF (V600A) monoclonal antibody (Cat. #26199).
Publications:
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review
    Seminars in Diagnostic Pathology