Anti-Abl (Y253H) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-Abl (Y253H) Mouse Monoclonal Antibody

Anti-Abl (Y253H) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-Abl (Y253H) Mouse Monoclonal Antibody

英文名称： Anti-Abl (Y253H) Mouse Monoclonal Antibody

产品编号： 26154

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IF、IHC

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Abl (Y253H) [26154]
[100 µL]		  

Catalog Number:  26154
Gene Symbol:  ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl
Description:   Anti-Abl (Y253H) Mouse Monoclonal Antibody
Background:   V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.
Immunogen:   A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin.
Applications:   ELISA, WB, IHC, IF
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IF: 1:50-1:100 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes Abl Y253H, but not wildtype of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Abl (Y253H) and wildtype proteins. Purified His-tagged Abl (Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-Abl (Y253H) mouse monoclonal antibody (Cat. #26154).
Immunofluorescence:  
Immunofluorescence of cells expressing Abl proteins with anti-Abl (Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl (Y253H) plasmid (right column), then fixed and stained with anti-Abl (Y253H) monoclonal antibody (Cat. #26154).